Figure 1. Overall experimental design. The two experimental groups included either sham treatment (PBS injection) or Israeli Acute Paralysis Virus (IAPV) inoculations via injection. At each investigated time point (5, 20, or 48 h post-injection) three biological replicates were collected, each consisting of one whole pupa that was individually processed for further transcriptomic and methylomic analyses.
Li-Byarlay H, Boncristiani H, Howell G, Herman J, Clark L, Strand MK, Tarpy D, Rueppell O, 2020. Transcriptomic and epigenomic dynamics of honey bees in response to lethal viral infection. Frontiers in genetics. 11, 1056